Table 1 Inclusion and Exclusion criteria employed in the present systematic mapping

Type of study

Original articles, studies presented in theses and conferences

Books, chapters, letters to editor, review studies (systematic reviews, meta-analysis), modelling studies that didn´t take environmental samples

Language

English

Non-English papers

Population

Lotic water bodies (rivers and some of its synonyms as: stream, watershed, catchment, basin, watercourse, waterway, brook, tributary, channel, creek, etc.) where the use of environmental DNA was applied

Wastewater Treatment Plants, sewage, lakes, microcosm experiments, estuarine or marine systems

Intervention/exposure

Use of environmental DNA (eDNA) framework/technique for studying rivers

There was no use of environmental DNA framework/technique

Outcome

Report of eDNA persistence, distribution, comparison of techniques where eDNA is used, characterization of community composition or the presence/absence of some species via eDNA use

No report of eDNA persistence, distribution, comparison of techniques where eDNA is used, characterization of community composition or the presence/absence of some species via eDNA use. Studies in which there was only a Draft Genome Complete Genome Sequencing Isolated

Study design

Experimental studies that included sampling of eDNA through some environmental matrix as: water, sediment or biofilm

–

Study designs that used eDNA for modelling persistence, resistance or distribution of eDNA

Studies that used eDNA for detection of species focusing in monitoring species at risk, exotic/invasive species, or those which have an important role on human health

Studies that compare the use of eDNA versus conventional techniques for monitoring and identify organisms

Studies that focus on determine a baseline for biodiversity of a lotic water body

Geography

There was no limitation for geographic areas

–

Period

There was no time limit for studies

–