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Table 2 Information to be extracted from the papers qualifying for inclusion in the review and to be stored in the CADIMA database

From: Are soil microbial endpoints changed by Bt crops compared with conventional crops? A systematic review protocol

Variable name

Definition

Geo-reference, longitude (field)

GPS coordinate WGS 84

Geo-reference, latitude (field)

GPS coordinate WGS 84

Location

Location of the experimental site

Crop

Name of the crop

GM Event

Name of the GM event

Treatment property

Type of Bt toxin(s) or comparator

Gene stacking

Information about stacked event , name of the stacked genes

Variety and cultivar

The commercial name of the variety and cultivar ID

Comparator properties

Information about the used comparator in relation with the Bt variety - isogenic or other.

Pesticide treatment(s)

Information about pesticide treatment , product name, active substance, amount, number, time and method for application.

Fertilizer use

Amount of NPK

Weather conditins

Mean annual temperature and precipitation

Experimental setup

Field; glasshouse; microcosmos; mesocosmos

Experimental design

Randomized complete blocks; Completely randomized; Multi location; Other. Kind of micro-mesocosmos

Plot size (field)

Calculation of the plot size (in square meters)

Plots number (field)

Number of plots per treatment

Cropping system (field)

Conventional; Reduced tillage; Conservation tillage etc.

Crop rotation (field)

Information about the history of the experimental field

Tillage date (field)

Date since last tillage event, including ploughing, harrowing, rotovation etc.

Date of cropping system establishment (field)

Date when the Bt crop was planted for first time

Seeding date (field)

Date of seeding within each growing season

Sampling date (field)

Date when the sampling was performed

Plant development stage

Stage at the point of sampling

Size (Micro-mesocosmos)

Size of the Experimental setup

Number of replicates (Micro-mesocosmos)

Number of replicates per treatment

Duration (Micro-mesocosmos)

Duration of the experiment

Temperature (Micro-mesocosmos)

Temperature at which the experiment was carried out

Other (Micro-mesocosmos)

Other informations related to experiments in Micro-mesocosmos

Data type

Of the value of the measured quantity, real, integer, binary, categorical, other

Method(s)

Short description of the applied method(s)

Microbial biomass

Measured biomass

Bacterial abundance

Number of bacteria, total and/or specific groups

Fungal abundance

Number of fungi, Total and/or specific groups

Microbial diversity

Taxonomic, genetic and/or functional diversity

Microbial activity

Specific activity measures, includes respiration, enzymatic activities, mineralisation etc.

Other microbial measurers

Other microbial measurements, including colonization of roots by mycorrhizal fungi, and root nodules

Sample depth (field)

Depth in the soil where the sample was taken

Sample amount

Amount of soil in one sample

Sampling location (field)

Location from which the samples are take, e.g. between rows, within rows, distance from roots

Extraction method

Used technique for extraction of microorganisms, the activity or DNA etc.

Sample size (field)

Sample size as reported by the author

Variability measure

STD; SEM; CLM; Variance

Quantity

Quantified variability

Soil type

Soil type according to WRB classification

Other fixed and random effects

E.g. fertilization, fungicide use, additional factors and effects of the experimental design

Data origin

Table or figure from which the data originates

Data extraction

How data was extracted, e.g. as exact number from tables or scaled numbers from graphs

Statistical analysis

Description of the statistical methods for analysis of variance

Reference

Bibliographic reference code as found in the GRACE CADIMA database (Central Access Database for Impact Assessment of Crop Genetic Improvement Technologies)

Source of funding

Description of the funding source of the study

Authors affiliation

Type of institution to which the first author belongs

Comments

Any other information which may be relevant

Keywords

Keywords for finding the reference in a systematic review - {list of keywords}