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Table 6 List of variables to be extracted from each relevant study

From: Are ladybird beetles (Coleoptera: Coccinellidae) affected by Bt proteins expressed in genetically modified insect-resistant crops? A systematic review protocol

Category Variable name Definition Type Closed terms
Study details study_id Unique identifier assigned to each study Integer No
study_type_ia Type of study I Integer Yes
study type_iib Type of study II Integer Yes
author Author(s) of the study String No
publication_year Year of publication Integer No
title Title of the study String No
citation Journal name, volume and page numbers String No
author_affiliation Type(s) of institutions that the author(s) are affiliated with (academic/private sector/government) String Yes
peer_reviewed Indicates whether the study was published in a peer-reviewed journal Yes/no Yes
study_funding Information on funding source of the study (public/private/mixed) String Yes
Test substance test_substance_ category Category of the test substance tested (e.g. purified protein, pollen, leaf) String Yes
bt_protein Bt protein tested String Yes
target Insect order targeted by the Bt protein (Lepidoptera/Coleoptera) String Yes
bt_protein_purityd Purity of the Bt protein tested in % Real No
bt_protein_ concentration Concentration of the Bt protein tested Real No
bt_protein_concentration_unit Unit of measurement for the Bt protein concentration String Yes
bt_protein_ equivalenced Indicates whether the microbially-produced/purified Bt protein tested is equivalent to the Bt protein produced by the GM plant (direct or indirect evidence) Yes/no Yes
bt_protein_equivalence_detailedd More detailed description on Bt protein equivalence String No
bt_protein_ stabilityd Indicates whether the microbially-produced/purified Bt protein tested was stable during the bioassay (direct or indirect evidence) Yes/no Yes
bt_protein_ stability_detailedd More detailed description on Bt protein stability String No
bt_protein_ bioactivityd Indicates whether the microbially-produced/purified Bt protein tested was biologically active (direct or indirect evidence) Yes/no Yes
bt_protein_ bioactivity_detailedd More detailed description on Bt protein activity String No
cropc Crop used in the study String Yes
eventc GM event of the crop tested String Yes
bt_hybrid_or_varc Bt hybrid or variety name String No
non-bt_hybrid_or_ varc Non-Bt hybrid or variety name String No
genetic_relatednessc Information on the relatedness between the Bt and the non-Bt line used in the study String No
Test organism ladybird_genus Ladybird taxonomic genus String Yes
ladybird_species Ladybird taxonomic species String Yes
life_stage_tested Life stage tested (larvae/adult) String Yes
Study design study_duration Duration of the study in days Real No
study_duration_ detailed More detailed information on study duration String Yes
negative_control Information on the negative (non-Bt) control used in the study String No
positive_controld Indicates whether a positive control was used Yes/no Yes
prey_speciese Prey taxonomic species String Yes
prey_species_ susceptibilitye Indicates whether prey is susceptible to the test substance Yes/no Yes
prey_species_ susceptibility_ evidencee Type of direct or indirect evidence provided to claim susceptibility of the prey species to the test substance String No
exposure_to_bt_ protein Indicates whether exposure of test species to Bt protein was confirmed Yes/no Yes
exposure_to_bt_ protein_detailed More detailed information on exposure to Bt protein String No
endpoint_ measured Endpoint measured in the study String Yes
endpoint_ measured_ unit Unit of measurement for the endpoint measured String Yes
endpoint_measured_ detailed Detailed description of endpoint measured String No
control_sample_size Sample size for the control (non-Bt) treatment Real No
exp_sample_size Sample size for the experimental (Bt) treatment Real No
stat_test_used Statistical test used by the author(s) String Yes
Results control_mean Mean for the control treatment Real No
exp_mean Mean for the experimental treatment Real No
control_std_err Standard error for the control treatment Real No
exp_std_err Standard error for the experimental treatment Real No
control_std_dev Standard deviation for the control treatment Real No
exp_std_dev Standard deviation for the experimental treatment Real No
data_location Figure, table or page number where means and variation were found String No
was_data_scanned Indicates whether figures were scanned to obtain data values String Yes
significant_dif_ observed Indicates whether a statistically significant effect was identified by the authors Yes/no/na Yes
Other Comments Space for comments for this record String No
  1. Bt: Bacillus thuringiensis; GM: genetically modified; na: not available
  2. aLaboratory, extended laboratory, glasshouse
  3. bType 1: direct and purified Bt protein; Type 2: direct and Bt plant; Type 3: indirect (prey-mediated) and purified Bt protein; Type 4: indirect (prey-mediated) and Bt plant
  4. cOnly relevant for studies with Bt plant material
  5. dOnly relevant for studies with purified Bt proteins
  6. eOnly relevant for indirect (prey-mediated) studies