General bias based on Bilotta et al. [32]
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Selection bias (Inadequate randomisation)
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No randomisation (site/tree selection based on availability, expert judgement or results)
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Randomisation (site/tree selection based on random number generator/tossing a coin)
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Performance and detection bias (researcher bias)
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No blinding/blinding broken/insufficient information
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Blinding done
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Reporting bias (incomplete results (variables))
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Not all pre-specified outcomes reported; measurements/methods for some outcomes were not pre-specified; incomplete reporting of one or more outcomes; not reporting on an expected outcome
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Study protocol is available and pre-specified outcomes are reported on; study protocol not available but all expected outcomes are reported on
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Attrition bias (incomplete results (observations))
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Resulting data points lower than expected from the methodology; no reason given for missing data related to outcome; bad imputation
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Reported data points correspond to methodology; data omission or imputation well documented and justified
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Adapted criteria
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Number of spatial or temporal replications (experimental unit) and observations [33]
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Spatial replication (experimental units)
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2 fields (widespread application)
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> 2 fields
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Or 2 rows (row-based installation)
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> 2 rows
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Or 2 trees (targeted application)
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> 2 trees
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OR
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Temporal replication
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1 frost night (temporal replication temperature change)
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> 1 frost night
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1 year (temporal replication yield/damage)
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> 1 year
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Observations (assessment level) per experimental unit
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< 4 trees (widespread or row-based)
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≥ 4 trees
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< 4 branches (targeted application)
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≥ 4 branches
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Baseline of test and control groups compared
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At least one of the following differs or is not described: management (pruning), soil type, topographic position, differences in cultivar and/or rootstock, orchard age
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All of the following factors are comparable: management (pruning), soil type, topographic position, differences in cultivar and/or rootstock, orchard age
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Location of temperature sensor given
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Closest meteorological station > 10 km away; no detail given
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Local sensors on field and beside flowers at several tree heights (lower, middle and upper position in tree)
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Reporting method
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Mean effects per experiment only
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Raw data, measure of variation
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External validity
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Cold chamber experiment
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Field experiment
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